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tev protease expression construct  (Addgene inc)


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    Addgene inc tev protease expression construct
    Figure 5. Representative results of purification and bioactivity screening (A) SDS-PAGE of FGF2 purification. E – Elution; <t>+TEV</t> – 2nd Ni-NTA flowthrough collected after <t>TEV</t> <t>protease</t> cleavage. The shift in size of the band is due to the removal of the 6His and TEV recognition sequence. (B) Western blot analysis of cell extracts treated with FGF2. C – commercial FGF2; NC – negative control – no FGF2; 1–5 – FGF2 orthologs; PC – positive control – 10% FBS. Panel B was adapted from1.
    Tev Protease Expression Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tev protease expression construct/product/Addgene inc
    Average 93 stars, based on 8 article reviews
    tev protease expression construct - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture."

    Article Title: Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture.

    Journal: STAR protocols

    doi: 10.1016/j.xpro.2023.102351

    Figure 5. Representative results of purification and bioactivity screening (A) SDS-PAGE of FGF2 purification. E – Elution; +TEV – 2nd Ni-NTA flowthrough collected after TEV protease cleavage. The shift in size of the band is due to the removal of the 6His and TEV recognition sequence. (B) Western blot analysis of cell extracts treated with FGF2. C – commercial FGF2; NC – negative control – no FGF2; 1–5 – FGF2 orthologs; PC – positive control – 10% FBS. Panel B was adapted from1.
    Figure Legend Snippet: Figure 5. Representative results of purification and bioactivity screening (A) SDS-PAGE of FGF2 purification. E – Elution; +TEV – 2nd Ni-NTA flowthrough collected after TEV protease cleavage. The shift in size of the band is due to the removal of the 6His and TEV recognition sequence. (B) Western blot analysis of cell extracts treated with FGF2. C – commercial FGF2; NC – negative control – no FGF2; 1–5 – FGF2 orthologs; PC – positive control – 10% FBS. Panel B was adapted from1.

    Techniques Used: SDS Page, Sequencing, Western Blot, Negative Control, Positive Control



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    Figure 5. Representative results of purification and bioactivity screening (A) SDS-PAGE of FGF2 purification. E – Elution; <t>+TEV</t> – 2nd Ni-NTA flowthrough collected after <t>TEV</t> <t>protease</t> cleavage. The shift in size of the band is due to the removal of the 6His and TEV recognition sequence. (B) Western blot analysis of cell extracts treated with FGF2. C – commercial FGF2; NC – negative control – no FGF2; 1–5 – FGF2 orthologs; PC – positive control – 10% FBS. Panel B was adapted from1.
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    Image Search Results


    Figure 5. Representative results of purification and bioactivity screening (A) SDS-PAGE of FGF2 purification. E – Elution; +TEV – 2nd Ni-NTA flowthrough collected after TEV protease cleavage. The shift in size of the band is due to the removal of the 6His and TEV recognition sequence. (B) Western blot analysis of cell extracts treated with FGF2. C – commercial FGF2; NC – negative control – no FGF2; 1–5 – FGF2 orthologs; PC – positive control – 10% FBS. Panel B was adapted from1.

    Journal: STAR protocols

    Article Title: Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture.

    doi: 10.1016/j.xpro.2023.102351

    Figure Lengend Snippet: Figure 5. Representative results of purification and bioactivity screening (A) SDS-PAGE of FGF2 purification. E – Elution; +TEV – 2nd Ni-NTA flowthrough collected after TEV protease cleavage. The shift in size of the band is due to the removal of the 6His and TEV recognition sequence. (B) Western blot analysis of cell extracts treated with FGF2. C – commercial FGF2; NC – negative control – no FGF2; 1–5 – FGF2 orthologs; PC – positive control – 10% FBS. Panel B was adapted from1.

    Article Snippet: Transform TEV protease expression construct (pTEVSH – Addgene: 125194. ) into BL21 (DE3) RIL competent cells. a. Thaw chemically competent BL21-CodonPlus (DE3)-RIL cells on ice. b. Thaw pTEVSH plasmid.

    Techniques: SDS Page, Sequencing, Western Blot, Negative Control, Positive Control